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Enzymes located in the inner matrix of mitochondria (in eukaryotes)
Note: Number of mitochondria per cell = activity of the cell. On average 1000 per cell (between 20 and 10 000)
Step 1: Synthesising ACETYL COENZYME A:
Step 2: Oxidising Acetyl CoA:
The Kreb's Cycle was discovered by using inhibitors (e.g. malonate) and isolating enzymes from tissues rich in mitochondria (e.g. bird flight muscle).
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