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Respiration Index

Introduction : Energy in Food
Glycolysis
Kreb's Cycle
The Electron Transport Chain
Anaerobic Respiration
Photosynthesis and Respiration

Topic Chapters Index

 

  

RQ

Carbohydrates

1.0

Fats

0.7

Proteins

0.8

Anaerobic respiration

>>1.0

 

RESPIRATION

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The Respiratory Quotient (RQ)

The rate of CO2 produced : The rate of O2 consumed.

 

C6H12O6 + 6O2 Arrow right 6CO2+ 6H2O   RQ = 6/6 = 1.0

C18H34O2 + 25.5O2 Arrow right18CO2 + 17H2O  RQ = 18/25.5 = 0.7

If photosynthesis is not controlled the RQ can be negative. Therefore, RQ measurements are taken in the dark.

 

Respirometers

These measure the rates of respiration in tissues or whole organisms.

KOH or NaOH absorb CO2

As CO2 is produced in the respirometer it is absorbed

= reduced pressure

= rate of respiration

But at the same time O2 is absorbed by the organism. This may be absorbed at different rates depending upon the food being consumed.

 

Two experiments are needed

(a) with KOH = CO2 (out)

(b) without KOH = CO2(out) O2 (in)

If glucose is used (b) will show no change (RQ = 1)

If fats are being used a reduced volume will be observed in (b) (RQ = 0.7).

If anaerobic respiration takes place an increase in volume will be observed in (b) (RQ >1)

Therefore: O2 (in) = Volume change in (a) - Volume change in (b)

Note: Ambient air pressure and temperature need to be controlled as they will also affect the volumes in the respirometer.

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