The Open Door Web Site
Classifying organisms according to their carbon source and energy supplies
The origin of oxygen in photosynthesis
Van Neil 1932 Comparing the biochemistry of autotrophs
Photosynthetic sulphur bacteria use H2S as their source of hydrogen
This suggested that in green plants the oxygen originates from the water molecule
Ruben: 1941: Confirmed this hypothesis using the heavy isotope 18O and mass spectrometry.
Hill: 1937: Using chloroplasts in vitro
Studying redox reactions in photosynthesis using artificial electron acceptors
The light dependent reaction involves the splitting of water. The electrons released are used to reduce electron acceptors. (This became known as the Hill Reaction)
Arnon 1954: The Hill reaction using natural electron acceptors
Arnon had effectively separated the light dependent reaction, which produces ATP, NADPH + H+ and oxygen, from the light independent reaction, which produces sugars.
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