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Powerpoint Presentation: Light Dependant Reaction

Photosynthesis Index

Classifying organisms according to their carbon source and energy supplies
Photosynthesis : The Development of Ideas
The light dependent reaction : Summary
Chlorophyll in the chloroplast
Chlorophyll and photosynthesis
Carbon fixation : The light independent reaction
Photosynthesis overview

Topic Chapters Index

 

Natural Electron ACCEPTORS

Pyridine nucleotides:

equation

Nicotinamide Adenine Dinucleotide (NAD) used in respiration in mitochondria,

Nicotinamide Adenine Dinucleotide Phosphate (NADP) used in photosynthesis in chloroplasts

Flavine Adenine Dinucleotide (FAD) also used in respiration

Ferredoxin the most difficult to reduce (and most easily oxidised)

Cytochromes: Conjugate proteins which contain a haem group.

The iron atom undergoes redox reactions

EQUATION

NB The iron atom in the haem group of haemoglobin does not go through a redox reaction. Haemoglobin is oxygenated or deoxygenated.

PHOTOSYNTHESIS

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The origin of oxygen in photosynthesis

Van Neil 1932 Comparing the biochemistry of autotrophs

Photosynthetic sulphur bacteria use H2S as their source of hydrogen

This suggested that in green plants the oxygen originates from the water molecule

Ruben: 1941: Confirmed this hypothesis using the heavy isotope 18O and mass spectrometry.

Hill: 1937: Using chloroplasts in vitro

Studying redox reactions in photosynthesis using artificial electron acceptors

 

 

 

 

Conclusion

The light dependent reaction involves the splitting of water. The electrons released are used to reduce electron acceptors. (This became known as the Hill Reaction)

 

Arnon 1954: The Hill reaction using natural electron acceptors

 

 

 

 

 

Arnon had effectively separated the light dependent reaction, which produces ATP, NADPH + H+ and oxygen, from the light independent reaction, which produces sugars.

 

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