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Biochemistry: Enzyme Index
Factors that effect Enzyme Activity
The effect of pH
pH levels will produce denaturation.
At pH values slightly different from the enzyme’s optimum value, small changes in the charges of the enzyme and its substrate molecules will occur. This change in ionisation will affect the binding of the substrate with the active site.
Note: The pH at which the enzyme molecule’s charges are equal is called its isoelectric point. This corresponds to its optimum pH. On an electrophoresis gel the enzyme would not move as it has no overall charge at this pH.
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